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È¥ÇÕÄ¡¿­±â ¾î¸°ÀÌ¿¡¼­ Streptococcus mutans¿Í Streptococcus sobrinusÀÇ ºÐÆ÷µµ Á¶»ç

PREVALENCE OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SOBRINUS IN CHILDREN WITH MIXED DENTITION

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ÀÌ¸í¼º, ÃÖ¼ºÃ¶, ¹ÚÀçÈ«,
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ÀÌ¸í¼º ( Lee Myung-Sung ) - °¡À̵åÄ¡°úº´¿ø
ÃÖ¼ºÃ¶ ( Choi Sung-Chul ) - °æÈñ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç ¹× ±¸°­»ý¹°Çבּ¸¼Ò
¹ÚÀçÈ« ( Park Jae-Hong ) - °æÈñ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç ¹× ±¸°­»ý¹°Çבּ¸¼Ò

Abstract

º» ¿¬±¸´Â 125¸íÀÇ 6-11¼¼ È¥ÇÕÄ¡¿­±â ¾Æµ¿À» ´ë»óÀ¸·Î ¿¬·É¿¡ µû¶ó Ãʱâ È¥ÇÕÄ¡¿­±â A±º(6-8¼¼; 62¸í)°ú Èıâ È¥ÇÕÄ¡¿­±â B±º(9-11¼¼; 63¸í)À¸·Î ºÐ·ùÇÏ°í À¯Ä¡ÀÇ dfs, ¿µ±¸Ä¡ÀÇ DFS¸¦ ±â·ÏÇÑ ÈÄ Àڱؼº Ÿ¾×À» äÃëÇÏ¿© TYCSB ¹èÁö¿¡ ¹è¾çÇؼ­ S. mutans¿Í S. sobrinus¸¦ ±¸ºÐÇÏ¿´À¸¸ç PCRÀ» ½ÃÇàÇÏ¿© È®ÀÎÇÏ¿´´Ù. S. mutans¿Í S. sobrinusÀÇ ºÐÆ÷µµ¿Í Ä¡¾Æ¿ì½ÄÁõ°úÀÇ »ó°ü°ü°è¸¦ Á¶»çÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
1. S. mutans¿Í S. sobrinusÀÇ »ó°ü°è¼ö´Â A±º 0.70, B±º 0.50·Î µÎ ±º ¸ðµÎ¿¡¼­ ³ôÀº »ó°ü°ü°è¸¦ º¸¿´´Ù.
2. S. mutans¿Í Ä¡¾Æ¿ì½ÄÁõ°úÀÇ °ü°è´Â A±º(r=0.25)°ú B±º(r=0.34) ¸ðµÎ¿¡¼­ ¾à°£ÀÇ °ü·Ã¼ºÀÌ ³ªÅ¸³µ´Ù.
3. A±º¿¡¼­ S. sobrinus¿Í Ä¡¾Æ¿ì½ÄÁõ°£¿¡ »ó°ü°ü°è°¡ ¾ø´Â °ÍÀ¸·Î ³ªÅ¸³µÀ¸¸ç, B±º(r=0.21)¿¡¼­´Â ¹Ì¾àÇÑ »ó°ü°ü°è°¡ ¼º¸³µÇ¾ú´Ù.
4. S. mutans¿Í ³ªÀÌÀÇ »ó°ü°ü°è´Â A±º°ú B±º ¸ðµÎ¿¡¼­ °ü·Ã¼ºÀÌ ¾ø´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
5. S. sobrinus¿Í ³ªÀÌÀÇ »ó°ü°ü°è´Â A±º(r=0.32)¿¡¼­´Â ¾à°£ÀÇ °ü·Ã¼ºÀÌ ³ªÅ¸³µ°í, B±º¿¡¼­´Â °ü·Ã¼ºÀÌ ¾ø´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù.

Mutans streptococci have been reported to be implicated in dental caries. Of these streptococcal species, Streptcoccus mutans and Streptococcus sobrinus are most commonly found in human dental caries. Prevalence of these bacterial species in dental caries is found to be varied in different races and countries. Therefore, importance of these bacteria in dental caries remains to be determined The present study was performed to find out correlation S. mutans and S. sobrinus with dental caries in 125 Korean children with mixed dentition between 6 to 11 years of age. They were classified as group A(6-8 years) and group B(9-11 years) by age. For the study, stimulated saliva samples were collected from each subject. The vials containing saliva specimens were serially diluted (1:10) in saline and plated in duplicate on tryptone-yeast extract-cysteine with sucrose and bacitracin (TYCSB) for S. mutans and S. sobrinus. After genomic DNA was extracted from the samples, polymerase chain reaction (PCR) amplification was performed for identification using universal primers and specific primers to S. mutans and S. sobrinus. Data of microbial variables were compared to caries status of the subjects.
According to this study, the result were as follows :
1. S. mutans versus S. sobrinus were moderate positive linear correlated in both group A(r=0.70) and group B(r=0.50).
2. Between S. mutans and dental caries there were weak positive linear correlation in both group A(r= 0.25) and group B(r=0.34).
3. S. sobrinus versus dental caries were not correlated in group A but slightly correlated in group B(r=0.21).
4. Between S. mutans and age, there were not correlation in both group. 5. S. sobrinus versus age were weak correlated in group A(r=0.32) but not correlated in group B.

Å°¿öµå

È¥ÇÕÄ¡¿­±â;Ä¡¾Æ¿ì½ÄÁõ;Àڱؼº Ÿ¾×
Mixed dentition;S. mutans; S. sobrinus;Dental caries;Stimulated saliva

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